TIANGEN Multi PCR Kit is a kit specifically developed for multiplex PCR.
TIANGEN Multi PCR Kit provides Multi HotStart DNA Polymerase with a unique
Multi HotStart PCR buffer containing the novel synthetic Factor MP. Together with
optimized salt concentrations, this Factor stabilizes specifically bound primers
and enables efficient extension of all primers in the reaction without optimization.
Q-Solution, a novel additive that enables efficient amplification of "difficult"
(e.g., GC-rich) templates, is also supplied. This chemically modified HotStart
Taq DNA polymerase is inactive during reaction setup and only becomes active
after incubation at 95oC, preventing the formation of nonspecific products and
primer–dimers in initial and later PCR cycles. Multiplex assays can be set up at
room temperature and HotStart Taq DNA Polymerase in the master mix is then
activated by a 15-minute incubation at 95°C, which can be incorporated into any
existing thermal-cycler program.
■ Chemically Modified Polymerase: only becomes active after incubation at 95oC
for 15 min
■ High Performance: High specificity and sensitivity with a built-in hot start
■ High Sensitivity: Highly suited for many types of multiplex PCR applications
■ Convenience: Easy to use and cost-effective
Amplification of multiple DNA regions
Amplification complicated templates
Amplification and analysis with high specificity
Definition of Activity Unit：
One unit is defined as the amount of the enzyme required to catalyze the
incorporation of 10 nmoles of dNT P’s into an acid-insoluble form in 30 minutes at
74°C using hering sperm DNA as substrate.
Store at -20°C.